Part:BBa_K1555003:Experience

We restricted two biobricks in the 2014 Distribution Kits and obtained this measurement device successfully. Here are some statistics for this biobrick. To test the efficiency and conditions can induce this promoter, we incubated the E.coli which have Pvgb and GFP inserted in plasmid under 37℃ and sealed conditions, every two hours since inoculated tested the concentration of oxygen and the florescence intensity of E.coli to further dig up the relationship between the expression of Pvgb and O2 concentration. Some manipulation must be done on our raw data to get the result. Considering the change in E.coli population, we should first divide the fluorescence signal by E.coli concentration. An assumption is made that the change rate of Pvgb expression is influenced by O2 concentration. Because fluorescence signal is an accumulation signal since the experiment is carried out. The change rate of fluorescence is the derivation of fluorescence signal by time. Since the data points are few and scattered, we have to utilize interpolation to generate more data points between the data points we got from experiment, and here spline interpolation is used.

Then we plot the Change Rate – O2 concentration image and do a quadratic regression. 3σ principle is utilized to remove some abnormal data from our experiment.

After interpolation the data points are intensive enough, so that we use the difference between two interfacing points as the estimation of derivation at that point:

From the chart we can see that with the concentration of dissolved oxygen decreased, the rate of GFP expression increased and reached maximum at last. Except the radioactivehalf-life of GFP, the negative speed under high concentration of dissolved oxygen revealed that there might exist a mechanism to decompose green florescence protein.

Applications of BBa_K1555003

This device was used to measure the oxygen sensitivity of promoter vgb.

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